Human TLR8 Stable Cell Line

Name	Human TLR8 Stable Cell Line
Supplier	Novus Biologicals
Catalog	NBP2-26271
Category	Cell Line
Prices	$849.00
Sizes	1 vial
Gene	TLR8
Species	Human
Growth Mode	Adherent Morphology: Epithelial
Description	The TLR8 stable cell line can be used for TLR8 flow cytometric calibration and detection control as well as TLR8-dependent functional assays. TLR8 expression in this cell line has been validated by Western blotting (fig. 1) and flow cytometry (fig. 2). Functional activity of this cell line has been validated by the NF-kB/SEAPorter™ Assay Kit (NBP2-25286, fig. 3). Contents: 3~4 x 10^6 cells Biosafety Level: 2
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Product images

Western Blot: Human TLR8 Stable Cell Line [NBP2-26271] - analysis of TLR8 expression in the TLR8 stable cell line using an HA antibody (20 ug total protein/lane). Legend. Vect: Vector control stable cell line; TLR8: TLR8 stable cell line.

Flow Cytometry: Human TLR8 Stable Cell Line [NBP2-26271] - analysis of the TLR8 stable cell line. The assay was performed using the NF-kB SEAPorterTM Assay Kit. The Vector stable cell line and TLR8 stable cell line were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with Imidazoquinoline resiquimod R-848 for 24 h followed by SEAP assay.

Flow Cytometry: Human TLR8 Stable Cell Line [NBP2-26271] - analysis of TLR8 expression in the TLR8 stable cell line. Intracellular expression of TLR8 in TLR8 stable cells was analyzed by flow cytometry using an Alexa Fluor 647-conjugated TLR8 antibody and compared with the Vector stable cell line. ntracellular TLR Staining Flow Kit was used for this test.